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Acetate RegulationThank you very much for your responses. Since I have a particular interest with acid production of yeast, mainly acetate, I take to opportunity to submit a follow up question specific on trying to make sure I understand the regulation of acetate production. I've read a few articles on the topic and from what I think I understand, during anaerobic fermentations it's a cytosolic aldehyde dehydrogenase (ALD3) that seems responsible for acetate production, via NAD+ regeneration? For some...
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Methylene BlueDear Dr Cone- In the last fortnight of yeast you mentioned the methylene blue technique. I use methylene blue, but am not sure how long it lasts or what its failure mode is - does it make live cells look dead or dead ones look alive when it goes off? It's not easy to find so I'd like to use it as long as possible. - David Edge RESPONSES: My experience with Methylene blue is that it puts out with time and requires filtering. The protocol for producing the 0.01% w/v stain: Dissolve 0....
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Freezing Dry YeastI have always frozen my dried yeasts after purchase and before use. I have not noticed any detriment to the final product, but I seem to remember reading somewhere in that past year or so not to freeze dry yeast, perhaps even on a package. Is it acceptable to freeze dry yeast or is there some detriment to the practice? Cheers, Mike Dixon RESPONSE: Mike, You are right; we had stated in our old technical data sheet that dry yeast should not be frozen. However in the last 3 years we had a...
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